3.4.21.B28 evolution the enzyme belongs to the post-prolyl peptidase family 731043 3.4.21.B28 evolution the enzyme exhibits similar substrate specificity and properties compared to prolyl oligopeptidase, EC 3.4.21.26, the latter is specifically inhibited by Z-Pro-prolinal, while the fibroblast activation protein alpha is not. In contrast to prolyl oligopeptidase, fibroblast activation protein alpha is not expressed in normal adult tissues. Substrate specificity preferences among these sequences include Pro-Phe-Thr, which is strongly cleaved by prolyl oligopeptidase, and Pro-Tyr-Asp, which is strongly cleaved by fibroblast activation protein alpha. Pro-Phe/Tyr-Asp/Glu sequences are extensively cleaved by both prolyl oligopeptidase and fibroblast activation protein alpha, but neither enzyme exhibit substantial cleavage of Pro-Asp/Glu-Phe-Tyr 731808 3.4.21.B28 malfunction plasma from FAP-deficient mice exhibits slower than wild-type clotting times 754737 3.4.21.B28 malfunction siRNA knockdown of FAP-alpha in a glioma cell line shows decreased invasion through brain extracellular matrix proteoglycan brevican and denatured collagen 707629 3.4.21.B28 metabolism FAP-alpha is significantly increased in breast cancer patients with poor outcome and survival 731551 3.4.21.B28 additional information overexpression of FAP-alpha results in a reduction of phosphorylated focal adhesion kinase level in both MCF-7 cells and MDA-MB-231 cells cultured in normal media and serum-free media, while the growth of MDA-MB-231 cells and the adhesion and invasion ability of both MCF-7 cells and MDA-MB-231 cells are not dramatically influenced by FAP-alpha expression 731551 3.4.21.B28 physiological function fibroblast activation protein-alpha promotes ovarian cancer cell proliferation and invasion via extracellular and intracellular signaling mechanisms, role of the non-enzymatic activities of the enzyme, overview. The enzyme, together with integrin alpha3beta1 and the uPAR signaling complex, mediates cancer cell migration in the HO-8910PM cell line via the small GTPase Rac1. FAP?-mediated upregulation of p-ERK occurs in a time-dependent manner 731767 3.4.21.B28 physiological function in vitro, FAP-alpha promotes proliferation and inhibits migration of breast cancer cells, potentially by regulating the FAK pathway 731551 3.4.21.B28 physiological function the enzyme is implicated in the modulation of the bioavailability and thus the function of a number of biologically active peptides. It has the potential to modulate the paracrine signaling in the human Langerhans islets 753766 3.4.21.B28 physiological function the enzyme may contributes to the focal matrix-degrading capability of stromal invadopodia 752802