3.4.15.6 physiological function compartmentalized degradation of cyanophycin, with the first step, catalyzed by cyanophycinase, taking place in the heterocysts and the second step, catalyzed by a dipeptidase, taking place mainly in the vegetative cells -, 728700 3.4.15.6 physiological function targeted expression in the cytosol, ER, and apoplasm of Nicotiana benthamiana. Translocation to the chloroplast is not successful. CphE accumulates in high amounts in the cytosol, moderate levels are present in the ER, and the enzyme is nearly undetectable in the apoplasm. The production in the ER leads to an increased number and size of necroses compared with cytosolic expression. Enzyme shows high and robust enzyme activity, even moderate CphE concentrations are sufficient to degrade cyanophycin granule polypeptide in plant extracts 752565