2.8.4.4	physiological function	a knockout strain grows slightly more slowly in liquid culture in rich medium than wild-type and forms smaller colonies on solid rich medium. The mutant strain tolerates a slightly higher concentration of streptomycin	728655	
2.8.4.4	physiological function	proteins RimO and YcaO, a conserved protein of unknown function, dramatically impact the modification status of ribosomal protein S12. The YcaO protein is involved in beta-methylthiolation of S12 and its absence impairs the ability of RimO to modify S12. The Escherichia coli specific beta-methylthiolation likely occurs when S12 is assembled as part of a ribosomal subunit. A RimO knockout mutant under aerobic conditions exhibits a 2fold decrease in transcription levels for a subset of genes belonging to operons that require the fumarate and nitrate reductase regulator for transcriptional activation and at least one of the nitrate and nitrite transcriptional response regulators NarL and NarP	-, 728300