2.5.1.98 malfunction knock-out of pssM leads to the absence of the pyruvic acid ketal group at the subterminal glucose in the repeating unit of exopolysaccharide (shown by 13C and 1H nuclear magnetic resonance analysis) 715316 2.5.1.98 physiological function Rhizobium leguminosarum bv. viciae strain VF39 synthesizes an acidic exopolysaccharide, which is necessary to establish nitrogen-fixing symbiosis with Pisum sativum, Vicia faba, and Vicia sativa. This exopolysaccharide is a polymer of an octasaccharide repeating unit consisting of a backbone of two glucose and two glucuronic acid residues, a side chain of three glucose and one gaThe pssM gene encodes ketal pyruvate transferase, which is responsible for pyruvylation of subterminal glucose in the EPS repeating unit 715316