1.3.7.7 evolution cyanobacteria, algae, bryophytes, pteridophytes and gymnosperms use an additional, light-independent enzyme dubbed dark-operative Pchlide oxidoreductase for chlorophyll biosynthesis, besides a light-dependent enzyme, mechanisms of protochlorophyllide a reduction in photosynthetic organisms, ooverview 726526 1.3.7.7 evolution cyanobacteria, algae, bryophytes, pteridophytes and gymnosperms use an additional, light-independent enzyme dubbed dark-operative Pchlide oxidoreductase for chlorophyll biosynthesis, besides a light-dependent enzyme, mechanisms of protochlorophyllide a reduction in photosynthetic organisms, overview 726526 1.3.7.7 evolution protein-protein interaction surfaces for transition state complexes of DPOR and nitrogenase, using PDB ID code 1M34, analysis of catalytic differences and similarities between DPOR and nitrogenase, overview 726394 1.3.7.7 evolution the enzyme is involved in the biosynthesis of chlorophylls and bacteriochlorophylls in gymnosperm, ferns, algae, and photosynthetic bacteria 725877 1.3.7.7 malfunction a strain lacking DPOR contains about 25% of the wild-type level of photosystems PSII and PSI when cultivated under light-activated heterotrophic growth conditions. Deletion of the chlL gene abolishes activity of the DPOR enzyme. Absence of the chlL gene causes a further 20% decrease in Chl content and therefore the resulting (pCER:por)/Dpor/DchlL strain termed SynPORreg reaches only 60-70% of Chl content present in wild-type 726255 1.3.7.7 metabolism chlorophyll biosynthesis is catalyzed by two multi subunit enzymes; a light-dependent and a light-independent protochlorophyllide oxidoreductase 725926 1.3.7.7 metabolism protochlorophyllide reduction is a key regulatory step in Chl biosynthesis 726104 1.3.7.7 metabolism the three-subunit enzyme dubbed DPOR operates in the synthesis of Bchls a, b, and g 726526 1.3.7.7 metabolism two independent enzymes catalyze the reduction of protochlorophyllide to chlorophyllide, which is the penultimate step in chlorophyll biosynthesis. One is light-dependent NADPH:protochlorophyllide oxidoreductase and the second type is dark-operative protochlorophyllide oxidoreductase 726255 1.3.7.7 additional information dark-grown seedlings of Pinus mugo accumulate chlorophyll and its precursor protochlorophyllide 726104