3.5.99.7 evolution ACC deaminase producing plant growth promoting rhizobacteria (PGPR) are isolated from the rhizosphere of Triticum aestivum var. Lok-1 and identified using 16S rRNA gene sequence analysis. Isolates are evaluated for various direct and indirect plant growth promoting (PGP) traits. 38 ACC deaminase producing PGPR are isolated which belonged to 12 distinct genera and falling into four phyla gamma-proteobacteria, beta-proteobacteria, Flavobacteria and Firmicutes. Klebsiella sp. is the most abundant genera and followed by Enterobacter sp. -, 758082 3.5.99.7 evolution comparative analysis of 1-aminocyclopropane-1-carboxylate (ACC) deaminase in selected plant growth promoting rhizobacteria (PGPR). The sequence and phylogenetic analysis of ACCD producing PGPR species represents the common conserved domain belonging to the tryptophan synthase beta subunit-like PLP-dependent enzymes superfamily and closely related to each other. The predicted homology models of ACCD of PGPR have similar protein structure with similar folds often share similar function. This analysis represents the evolutionary conservation and same biochemical function of ACCD producing plant growth-promoting rhizobacteria -, 757505 3.5.99.7 evolution distribution of 1-aminocyclopropane-1-carboxylate deaminase genes among type species of the genus Methylobacterium, overview -, 746770 3.5.99.7 evolution library screening and genotyping of rhizosphere soli isolates, sequence comparisons and phylogenetic analysis, overview 755841 3.5.99.7 evolution phylogenetic, chemotaxonomic, phenotypic and genomic characterisation indicates that the detected strains belong to a distinct genus and species of the order Oceanospirillales for which the names Pokkaliibacter gen.nov., and Pokkaliibacter plantistimulans sp. nov., are proposed with L1E11 / DSM 28732 / MCC 2992 as the type strain. The isolate is found in association with plants grown in brackish environments and possesses plant growth promotion traits such asACC deaminase, siderophore production and phosphate solubilisation. The type species of the genus is Pokkaliibacter plantistimulans -, 758498 3.5.99.7 malfunction compared with the wild-type A1501, the acdS mutant A1815 is more sensitive to the environmental stresses of salt and heavy metal shock. The survival rate of A1815 is lowered by 1-2 orders of magnitude compared with the wild-type when exposed to 1.0 M NaCl treatment for 3 h and by 2-3 orders of magnitude when exposed to 13.2 mM NiCl2 for 3 h -, 757414 3.5.99.7 malfunction RNAi silencing of the ACCD gene in Trichoderma asperellum shows decreased ability of the mutants to promote root elongation of Brassica napus seedlings -, 712035 3.5.99.7 malfunction Sinorhizobium sp. BL3-enhancing ACC deaminase activity (BL3+) and defective mutant (BL3-) strains are constructed, modulation competitiveness is weaker in BL3- than in the wild-type, but is stronger in BL3+. The inoculation of BL3- into mung bean results in less plant growth, a lower nodule dry weight, and smaller nodule number than those in the wild-type, whereas the inoculation of BL3+ has no marked effects 757612 3.5.99.7 malfunction the acdS and lrpL double mutant strain Agrobacterium tumefaciens D3-1 has lost the ability to promote plant root elongation -, 719206 3.5.99.7 metabolism all the PGPR isolates register phosphate and zinc solubilization accompanied by drop in pH of the medium -, 758082