3.4.22.14	metabolism	active actinidin retains its primary structure and proteolytic activity after 2 h of simulated gastric digestion, followed by 2 h of intestinal digestion	734567	
3.4.22.14	additional information	the enzyme increases intestinal permeability in both in vivo (mice) and in vitro (Caco-2 cell culture) models. The food allergen with intrinsic proteolytic activity can compromise the gut epithelial barrier, contributing to the sensitization process in food allergy pathogenesis	752807	
3.4.22.14	physiological function	actinidin disrupts the epithelial barrier function of human intestinal T84 cells by degrading the tight junction protein occludin. Exposure of T84 cells to biologically active actinidin significantly decreases the transepithelial resistance across the intestinal epithelial monolayers	733900	
3.4.22.14	physiological function	actinidin is both immunogenic and allergenic at relatively low doses. Vigorous IgG and IgG1 antibody and high titre IgE antibody responses are recorded in response to actinidin	734050	
3.4.22.14	physiological function	actinidin is important in monoallergy to kiwifruit	708861