2.1.1.202	evolution	both the nucleotide position and percent methylation of tRNAs and rRNAs m5C sites are conserved across all species analysed	735864	
2.1.1.202	evolution	both the nucleotide position and percent methylation of tRNAs and rRNAs m5C sites are conserved across all species analysed, overview	735864	
2.1.1.202	evolution	both the nucleotide position and percent methylation of tRNAs and rRNAs m5C sites were conserved across all species analysed	735864	
2.1.1.202	evolution	Identification of m5C sites in nuclear, chloroplast and mitochondrial tRNAs. 39 cytosine 5-methylation sites are identified at 5 structural positions and are located in tRNA secondary structure at positions C38, C48, C49, C50 and C72, pattern of methylation in individual tRNA isodecoders, overview. Identification of single-nucleotide resolution of cytosine 5-methylation sites in non-coding ribosomal RNAs and transfer RNAs of all three subcellular transcriptomes across six diverse species. Both the nucleotide position and percent methylation of tRNAs and rRNAs cytosine 5-methylation sites are conserved across all species analysed, overview	735864	
2.1.1.202	evolution	phylogenetic tree of Trm4/ NSun2 homologues, overview. Enzyme Trm4a is responsible for in vivo methylation of C34 and C48 methylation, whereas Trm4b methylates C49 and C50, tRNAProCGG is the only tRNA methylated by both Trm4a and Trm4b. Schizosaccharomyces pombe shows an unusual separation of activities of the NSun2/Trm4 enzymes that are united in a single enzyme in other eukaryotes like humans, mice and Saccharomyces cerevisiae	-, 757846	
2.1.1.202	evolution	phylogenetic tree of Trm4/ NSun2 homologues, overview. In contrast to most other organisms, fission yeast Schizosaccharomyces pombe carries two Trm4/NSun2 homologues, Trm4a (SPAC17D4.04) and Trm4b (SPAC23C4.17). Enzyme Trm4a is responsible for in vivo methylation of C34 and C48 methylation, whereas Trm4b methylates C49 and C50, tRNAProCGG is the only tRNA methylated by both Trm4a and Trm4b. Schizosaccharomyces pombe shows an unusual separation of activities of the NSun2/Trm4 enzymes that are united in a single enzyme in other eukaryotes like humans, mice and Saccharomyces cerevisiae	-, 757846	
2.1.1.202	evolution	the enzyme belongs to the RsmF/YebU/NSUN2 family of cytosine 5-methylation-RNA methyltransferases utilizing two cysteines in their catalytic pocket	736856	
2.1.1.202	malfunction	autosomal-recessive loss of the NSUN2 gene is a causative link to intellectual disability disorders in humans. Loss of cytosine-5 methylation in vault RNAs causes aberrant processing into Argonaute-associated small RNA fragments that can function as microRNAs. Impaired processing of vault ncRNA may contribute to the etiology of NSun2-deficiency human disorders	735907	
2.1.1.202	malfunction	disruption of the ORF YBL024w leads to the complete absence of m5C in total yeast tRNA. No tRNA:m5C-methyltransferase activity towards all potential m5C methylation sites is detected in the extract of the disrupted yeast strain. The protein product of a single gene is responsible for complete m5C methylation of yeast tRNA	639471	
2.1.1.202	malfunction	exposure to oxidative stress efficiently repressed NSUN2, causing a reduction of methylation at specific tRNA sites. Loss of NSUN2 alters the biogenesis of tRNA-derived noncoding fragments (tRFs) in response to stress, leading to impaired regulation of protein synthesis. The intracellular accumulation of a specific subset of tRFs correlates with the dynamic repression of global protein synthesis	758103