6.2.1.13	evolution	the enzyme is a member of the NDP-forming acyl-CoA synthetase enzyme superfamily	744911	
6.2.1.13	malfunction	amoebae lacking enzyme activity are unable to reestablish the acetyl-CoA/CoA ratio after an oxidative stress challenge	744414	
6.2.1.13	malfunction	gradual ACS gene silencing (49-93%) significantly decreases the acetate flux without affecting the levels of glycolytic metabolites and ATP in trophozoites. Amoebae lacking ACS activity are unable to reestablish the acetyl-CoA/CoA ratio after an oxidative stress challenge	744414	
6.2.1.13	metabolism	the enzyme is related to the phosphate-dependent fermentative glycolysis in Entamoeba histolytica. The end-products of carbohydrate catabolism in the parasite are ethanol and acetate, produced by the bifunctional aldehyde-alcohol dehydrogenase (ADHE) and ACS, respectively, overview	744414	
6.2.1.13	metabolism	the main role of the enzyme in the parasite energy metabolism is to recycle CoA for glycolysis to proceed under aerobic conditions	744414	
6.2.1.13	additional information	residue His252 is essential for enzymatic activity, substrate binding structure and mechanism, overview	744911	
6.2.1.13	physiological function	Archaeoglobus fulgidus strain 7324 converts starch to acetate via a modified Embden-Meyerhof pathway and acetyl-CoA synthetase (ADP-forming)	-, 721449	
6.2.1.13	physiological function	growth arrest is caused by elevated Acs, EC 6.2.1.1, activity, while overproduction of ADP-forming Ac-CoA synthesizing systems do not affect the growth behaviour of acetylation-deficient or acetylation-proficient strains	716267