4.2.1.108 evolution ectoine biosynthetic genes (ectABC) are widely distributed in bacteria, ectoine synthase is a member of the cupin superfamily -, 746650 4.2.1.108 evolution phylogenetic analysis of nucleotides and amino acids reveals that the ectA, B and C sequences of Bacillus clausii NIOT-DSB04 were conserved in many eubacteria -, 756904 4.2.1.108 evolution the enzyme is a metal-containing member of the cupin superfamily. Cupins contain two conserved motifs: G(X)5HXH(X)3,4E(X)6G and G(X)5PXG(X)2H(X)3N (the letters in bold represent those residues that often coordinate the metal) -, 749081 4.2.1.108 malfunction in Halomonas elongata mutant strains deficient in ectA, encoding L-2,4-diaminobutyric acid acetyltransferase, no ectoine synthesis is obeserved -, 713911 4.2.1.108 metabolism ectoine synthase is involved in the biosynthetic pathway of L-ectoine and (S,S)-beta-hydroxyectoine, involvement of ectoine synthase in 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate biosynthesis in vitro -, 713911 4.2.1.108 metabolism synthesis of ectoine occurs from the intermediate metabolite L-aspartate-beta-semialdehyde and comprises the sequential activities of three enzymes: L-2,4-diaminobutyrate transaminase (EctB, EC 2.6.1.76), 2,4-diaminobutyrate acetyltransferase (EctA, EC 2.3.1.178), and ectoine synthase (EctC, EC 4.2.1.108) -, 749081 4.2.1.108 metabolism the enzyme is involved in ectoine biosynthesis -, 729687 4.2.1.108 metabolism the enzyme is involved in ectoine biosynthesis. Ectoine is a compatible solute, serves as a protective compound in many halophilic eubacterial cells under stress -, 756904 4.2.1.108 physiological function extraordinary protective properties of 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate produced by ectoine synthase -, 713911 4.2.1.108 physiological function microorganisms that carry the ectoine biosynthetic genes (ectABC) make copious amounts of ectoine as a cell protectant in response to high-osmolarity challenges. Ectoine synthase (EctC) is the key enzyme for the production of this compatible solute and mediates the last step of ectoine biosynthesis. It catalyzes the ring closure of the cyclic ectoine molecule -, 746650