2.8.1.6	malfunction	single mutations in the global regulator IscR can substantially improve cellular tolerance to BioB overexpression, increasing Escherichia coli dethiobiotin-to-biotin biocatalysis by more than 2.2fold. In the overexpression strains, FeS-cluster depletion is the main reason for toxicity. IscR mutations significantly affect cell viability and improve cell factories for de novo biosynthesis of thiamine by 1.3fold and lipoic acid by 1.8fold	
2.8.1.6	metabolism	biotin synthase catalyzes the final step in the biosynthesis of biotin	
2.8.1.6	physiological function	biotin synthase catalyses the final step in the biosynthesis of biotin	
2.8.1.6	physiological function	biotin synthase catalyses the last step in the biosynthesis of biotin	
2.8.1.6	physiological function	biotin synthase is an AdoMet radical enzyme that catalyses the final step in the biosynthesis of biotin	
2.8.1.6	physiological function	overexpression of biotin synthase is required for efficient generation of sulfur-35 labeled biotin in Escherichia coli. Coexpression of both BioB and the biotin ligase, BirA, is required for efficient biotinylation of biotin carboxy carrier protein-79	
2.8.1.6	physiological function	avirulent nematodes produce an active biotin synthase while virulent ones contain an inactive form of the enzyme. A diverse mixture of BioB alleles is found with the virulent form being the most prevalent. Complementation of an Escherichia coli vitamin B7 auxotroph only occurs for the bacteria containing the avirulentBioB allele containing the P24/R44 amino acid combination	
2.8.1.6	physiological function	a semistable intermediate in the formation of the first C-S bond is 9-mercaptodethiobiotin linked to a paramagnetic [2Fe-2S] cluster through one of its bridging sulfides. C6, the target of the second hydrogen-atom abstraction, is now in close proximity to the nascent thioether sulfur and is ideally positioned for the second C-S bond forming event