2.4.1.288 metabolism GlfT2 catalyzes the formation of mycobacterial galactan, mediating galactan polymerization using only one active site that manifests dual regioselectivity 2.4.1.288 physiological function GlfT2 is essential for mycobacterial cell wall biosynthesis 2.4.1.288 metabolism the enzyme catalyzes the addition of the third and subsequent Galf residues, but not the first two, to the lipid template in galactan synthesis 2.4.1.288 metabolism the enzyme catalyzes the formation of both beta-5-linked and beta-6-linked galactofuranoses and is responsible for the synthesis of the bulk galactan portion of AG 2.4.1.288 physiological function the enzyme mediates the cell wall galactan production through a sequence-specific polymerization 2.4.1.288 physiological function the free-energy barriers for beta-(1-6) and beta-(1-5) linkages are mainly the same, 29.6 kcal/mol for the beta-(1-6) reaction and 28.2 kcal/mol for the beta-(1-5) reaction. Both reactions occur at a single catalytic site with a SN2-type reaction mechanism with a dissociative oxocarbenium ion-like transition state for both the beta-(1-6) and beta-(1-5) reactions. In both reactions the furanose ring adopts an utterly flat ring in the transition state