2.1.1.274	evolution	the enzyme belongs to SABATH family, a class of O-methyltransferases and N-methyltransferases	736189	
2.1.1.274	evolution	the enzyme belongs to the protein family of SABATH methyltransferases, ten genes encode isozymes PaSABATH1-10. Five of the PaSABATH isozymes (PaSABATH3, PaSABATH6, PaSABATH7, PaSABATH8, and PaSABATH9) do not show activity with any of the four substrates, i.e. indole-3-acetic acid, jasmonic acid, giberellic acid A3, and salicylic acid, the other five of the PaSABATHs each show activity with one or more of the four substrates. PaSABATH1 has the highest level of specific activity with indole-3-acetic acid and is renamed as PaIAMT (EC 2.1.1.275). PaSABATH2 has the highest level of specific activity with salicylic acid and is designated as PaSAMT (EC 2.1.1.274). For comparison, PaSAMT is also assayed with two compounds of similar structure benzoic acid and anthranilic acid (cf. EC 2.1.1.273). While PaSAMT has no activity with anthranilic acid, its activity with benzoic acid is approximately 8% of that with salicylic acid. PaSABATH4, PaSABATH5 and PaSABATH10 show the highest level of specific activity with jasmonic acid and are renamed PaJAMT1, PaJAMT2, and PaJAMT3, respectively (EC 2.1.1.141). Their products are confirmed to be methyljasmonate	757941	
2.1.1.274	evolution	the enzyme belongs to the SABATH family, phylogenetic analysis and tree, detailed overview. Twenty-eight Populus SABATH genes are divided into three classes with distinct divergences in their gene structure, expression responses to abiotic stressors and enzymatic properties of encoded proteins. Populus class I SABATH proteins convert indole-3-acetic acid (IAA) to methyl-IAA, class II SABATH proteins convert benzoic acid (BA) and salicylic acid (SA) to methyl-BA and methyl-SA, while class III SABATH proteins convert farnesoic acid (FA) to methyl-FA. For Populus class II SABATH proteins, both forward and reverse mutagenesis studies show that a single amino acid switch between PtSABATH4 and PtSABATH24 results in substrate switch. Of the Populus SABATH class II proteins, PtSABATH4 and 24 show the highest activity towards SA and BA, respectively	757984	
2.1.1.274	malfunction	a knockout mutant fails to accumulate methyl salicylate following pathogen infection. These plants also fail to accumulate salicylate or its glucoside in the uninoculated leaves and do not develop systemic acquired resistance. However, the mutant exhibits normal levels of effector-triggered immunity and pathogen-associated molecular pattern-triggered immunity to Pseudomonas syringae and Hyaloperonospora arabidopsidis	726013	
2.1.1.274	malfunction	AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview	-, 757709	
2.1.1.274	malfunction	basal salicylic acid (SA) levels in Arabidopsis thaliana plants that constitutively overexpress PbBSMT compared with those in Arabidopsis wild-type Col-0 are reduced approximately 80% versus only a 50% reduction in plants overexpressing AtBSMT1. PbBSMT-overexpressing plants are more susceptible to Plasmodiophora brassicae than wild-type plants, they also are partially compromised in nonhost resistance to Albugo candida. In contrast, AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Furthermore, transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum cv. Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview	757709	
2.1.1.274	malfunction	for Populus class II SABATH proteins, both forward and reverse mutagenesis studies show that a single amino acid switch between PtSABATH4 and PtSABATH24 results in substrate switch. The mutation of Met156 to His results in a switch from a preference for salicylic acid (SA) over benzoic acid (BA) in wild-type PtSABATH4 to a preference for BA over SA in the M156H mutant. The mutation of His157 of PtSABATH24 (EC 2.1.1.273) to a methionine residue also results in a switch from a preference for BA over SA in wild-type PtSABATH24 to a preference for SA over BA in the H157M mutant. The PtSABATH4 mutation M314V results in decreased enzymatic activities towards both the substrates salicylic acid (SA) over benzoic acid (BA), but not in a substrate switch	757984	
2.1.1.274	malfunction	overexpression of LcSAMT gene markedly enhances the methylsalicylate (MeSA) content and reduces the accumulation of salicylate (SA) in transgenic tobacco plants, the conversion of MeSA from SA leads to the depletion of the free SA pool. Overexpression of LcSAMT gene in tobacco significantly increases sensitivity of transgenic plants to drought stress, probably due to the decreased SA accumulation. Increased accumulation of ROS, elevated MDA levels, reduced proline contents, and lowered expression of APX, CAT and SOD genes are also observed in the LcSAMT transgenic tobacco plants under drought stress, which means that the LcSAMT-overexpressing transgenic tobacco plants have decreased resistance to oxidative stress in comparison with control plants under drought stress. LcSAMT-overexpressing transgenic tobacco plants display decreased abscisic acid (ABA) accumulation and reduced transcript expression of NtNCED1 and NtRD22 genes. Therefore, the increased sensitivity of transgenic plants overexpressing LcSAMT gene to drought stress might also act through an ABA-dependent pathway. Overexpression of LcSAMT decreases RWC, proline, chlorophyll content, and the photosynthetic capacity, and increases MDA content of transgenic Nicotiana tabacum plants exposed to drought stress	756604	
2.1.1.274	malfunction	recombinant BSMT enzyme expression in Arabidopsis thaliana under the control of a dexamethasone-inducible promoter leads to chlorosis and altered host susceptibility. Transcription of PbBSMT is associated with: (1) strong leaf phenotypes from anthocyanin accumulation and chlorosis followed by browning, (2) increased plant susceptibility after infection with Plasmodiophora brassicae that is manifested as more yellow leaves and reduced growth of upper plant parts, and (3) induced transgenic plants are not able to support large galls and had a brownish appearance of some clubs. Microarray data indicate that chlorophyll loss is accompanied by reduced transcription of genes involved in photosynthesis, while genes encoding glucose metabolism, mitochondrial functions and cell wall synthesis are upregulated. Phenotype overview	757953	
2.1.1.274	metabolism	expression patterns of Populus SABATH genes under normal growth conditions and abiotic stress, overview	757984	
2.1.1.274	metabolism	indole-3-acetic acid (IAA), gibberellins (GAs), salicylic acid (SA) and jasmonic acid (JA) exist in methyl ester forms in plants in addition to their free acid forms. The enzymes catalyze methylation of these carboxylic acid phytohormones occurs in form of ten isozymes, PaSABATH1-10	757941	
2.1.1.274	metabolism	the enzyme is critical for methyl salicylate synthesis	726172	
2.1.1.274	metabolism	the enzyme is involved in the secondary metabolic pathways leading to the formation of scent volatiles in Jasminum sambac flower, overview. Developmental pattern of emission of sent volatiles in Jasminum sambac flower on a time-course basis, and concentrations of the above benzenoids and terpenes in the flowers with respect to spatial and temporal regulation	749017	
2.1.1.274	metabolism	the expression and activities of MeSA esterase (MES), benzoic acid/SA methyltransferase (BSMT) and starch synthase (SS 1) are presumed to be involved in the defense response and monitored. Specifically, BoMES2, BoMES4_2, BoMES9 genes might be involved in the esterase activity to form free salicylate, supporting their defense activity during fungal infection. Another gene potentially involved in the esterase activity during clubroot development is BoMES9_1. Analysis of protein interaction network, overview. BoBSMT1 shows interaction with UGT74F2 and DIR1, which can play positive regulatory roles in glucosyltransferase and SAR signaling respectively	756883	
2.1.1.274	additional information	analysis of molecular mechanism of LcSAMT gene	756604	
2.1.1.274	additional information	differences in susceptibility to Plasmodiophora brassicae are characterized based on presence or absence of root galls in the two lines of Brassica oleracea	756883	
2.1.1.274	additional information	three-dimensional structure modeling of PtSABATH4 based on the 1M6E crystal structure. Similar to 1M6E, residues Met156 and Met314 of PtSABATH4 also create a molecular clamp that encompasses the benzyl ring of salicylate	757984	
2.1.1.274	physiological function	both methyl salicylate and methyl jasmonate are essential for systemic resistance against Tobacco mosaic virus, possibly acting as the initiating signals for systemic resistance, irreplaceable roles of methyl salicylate and methyl jasmonate in systemic resistance response	736841	
2.1.1.274	physiological function	Brassica oleracea var. capitata production is severely affected by clubroot disease caused by the soil-borne plant pathogen Plasmodiophora brassicae. During clubroot development, methyl salicylate (MeSA) is biosynthesized from salicylic acid (SA) by salicylate methyltransferase. Methyl salicylate esterase (MES) plays a major role in the conversion of MeSA back into free SA. Analysis of the interrelationship between MES and salicylate methytransferases during clubroot development, overview	756883	
2.1.1.274	physiological function	enzyme benzoic acid/salicylic acid carboxyl methyltransferase is enzyme responsible for catalyzing benzoic acid and salicylic acid to methyl benzoate and methyl salicylate, respectively, and is involved in floral scent production from lily	736189	
2.1.1.274	physiological function	enzyme benzoic acid/salicylic acid carboxyl methyltransferase is enzyme responsible for catalyzing salicylic acid and benzoic acid via salicylic acid to methyl salicylate, and is involved in plant defense against pathogens. The phenylalanine ammonia-lyase, not the isochorismate pathway, is the primary route for salicylic acid production in tomato	736696	
2.1.1.274	physiological function	isoform SAM1 plays a role in soybean defence against the soybean cyst nematode Heterodera glycines. Enzyme overexpression also affects the expression of selected genes involved in salicylic acid biosynthesis and salicylic acid signal transduction	726129	
2.1.1.274	physiological function	isoform SAMT1 may play a dual regulation role of distinct signaling in Atropa belladonna plants, namely the signaling pathway of the SA-dependent response, and also a jasmonic acid dependent response in local regions	726132	
2.1.1.274	physiological function	mimicking the host regulation of salicylic acid: a virulence strategy by the clubroot pathogen Plasmodiophora brassicae, overview. The plant hormone salicylic acid (SA) plays a critical role in defense against biotrophic pathogens, e.g. Plasmodiophora brassicae, which is an obligate pathogen of crucifer species and the causal agent of clubroot disease of canola (Brassica napus), encoding a protein with very limited homology to benzoic acid (BA)/SA-methyltransferase, designated PbBSMT. Enzyme PbBSMT is an effector, which is secreted by Plasmodiophora brassicae into its host plant to deplete pathogen-induced SA accumulation. Plasmodiophora brassicae uses PbBSMTto overcome SA-mediated defenses by converting SA into inactive methyl salicylate (MeSA). PbBSMT suppresses local defense and provide evidence that PbBSMT is much more effective than endogenous Arabidopsis thaliana host enzyme AtBSMT1 at suppressing the levels of SA and its associated effects. PbBSMT is much more effective than AtBSMT1 at both reducing endogenous and exogenous SA levels and at suppressing multiple levels of resistance, including nonhost and basal resistance as well as pattern-triggered immunity (PTI) and effector-triggered immunity (ETI)	757709	
2.1.1.274	physiological function	mimicking the host regulation of salicylic acid: a virulence strategy by the clubroot pathogen Plasmodiophora brassicae, overview. The plant hormone salicylic acid (SA) plays a critical role in defense against biotrophic pathogens, e.g. Plasmodiophora brassicae, which is an obligate pathogen of crucifer species and the causal agent of clubroot disease of canola (Brassica napus). A pathogen salicylate methyltransferase, PbBSMT, suppresses local defense and provide evidence that PbBSMT is much more effective than endogenous Arabidopsis thaliana host methyltransferase enzyme AtBSMT1 at suppressing the levels of SA and its associated effects. PbBSMT is much more effective than AtBSMT1 at both reducing endogenous and exogenous SA levels and at suppressing multiple levels of resistance, including nonhost and basal resistance as well as pattern-triggered immunity (PTI) and effector-triggered immunity (ETI)	-, 757709	
2.1.1.274	physiological function	overexpression of isoform BSMT1 compromises systemic acquired resistance and pathogen-associated molecular pattern-triggered immunity but not effector-triggered immunity	726013	
2.1.1.274	physiological function	salicylic acid (SA) is a phenolic compound involved in plant growth and development. Salicylic acid carboxyl methyltransferase (SAMT) can catalyze the methylation of SA with S-adenosyl-L-methionine as the methyl donor to form methyl salicylate (MeSA). Recombinant salicylic acid carboxyl methyltransferase-like gene LcSAMT from Lycium chinense negatively regulates the drought response in transgenic tobacco. Enzyme LcSAMT regulates the expression of stress-related genes in transgenic Nicotiana tabacum plants exposed to drought stress	756604	
2.1.1.274	physiological function	salicylic acid carboxyl methyltransferase activity from Camellia sinensis provides the aroma compound methyl salicylate (MeSA) during the withering process of white tea. During the withering process for white tea producing, MeSA is generated by salicylic acid carboxyl methyltransferase (SAMT) with salicylic acid (SA), and the specific floral scent is formed	757035	
2.1.1.274	physiological function	the enzyme is involved in enzymatic production and emission of floral scent volatiles in Jasminum sambac	749017	
2.1.1.274	physiological function	the obligate biotrophic pathogen Plasmodiophora brassicae causes clubroot disease in Arabidopsis thaliana, which is characterized by large root galls. Salicylic acid production is a defence response in plants, and its methyl ester is involved in systemic signalling. Plasmodiophora brassicae suppresses the plant defence reactions via its methyltransferase, PbBSMT with homology to plant methyltransferases. The PbBSMT gene is maximally transcribed when salicylic acid production is highest, and enzyme PbBSMT can methylate salicylic acid, benzoic and anthranilic acids. Plasmodiophora brassicae secretes enzyme PbBSMT into the host cell, where it methylates the defence signal salicylate. The resulting methyl salicylate fails to upregulate plant defence reactions and is transmitted to leaves, where it is emitted or converted back to salicylate	736842	
2.1.1.274	physiological function	the plant pathogenic protist Plasmodiophora brassicae causes clubroot disease of Brassicaceae. The biotrophic organism can downregulate plant defence responses via its salicylic acid methyltransferase. The enzyme is involved in attenuation of host defence responses in the roots by metabolising a plant defence signal. Role for the methylation of salicylic acid in attenuating plant defence response in infected roots as a strategy for intracellular parasitism. Salicylic acid (SA) is a plant defence hormone that acts as a prominent signal in response to biotrophic pathogens	757953