2.1.1.182	evolution	the KsgA family belongs tothe group of S-adenosyl-L-methionine-dependent methyltransferases, known as class I MTases, KsgA is related to DNA adenosine methyltransferases, which transfer only a single methyl group to their target adenosine residue. Part of the discrimination between mono- and dimethyltransferase activity lies in a single residue in the active site, L114; this residue is part of a conserved motif, known as motif IV, which is common to a large group of S-adenosyl-L-methionine-dependent methyltransferases	718932	
2.1.1.182	malfunction	acquisition of high-level resistance to kasugamycin at an extraordinarily high frequency, ksgA mutants display a disadvantage in overall fitness compared to the parent strain	701730	
2.1.1.182	malfunction	cold sensitivity and altered ribosomal profiles are associated with a DELTAksgA genotype in Escherichia coli	705774	
2.1.1.182	malfunction	loss of this dimethylation confers resistance to the antibiotic kasugamycin	-, 705141	
2.1.1.182	malfunction	mutants which contain a frameshift mutation in ksgA are severely impaired for growth	-, 702903	
2.1.1.182	malfunction	spontaneous KSGR mutants in Neisseria gonorrhoeae arise through mutations in ksgA, which are likely to reduce KsgA activity and lead to undermethylated rRNA and thus resistance to kasugamycin	704036	
2.1.1.182	malfunction	strains lacking the methylase are resistant to kasugamycin	705860	
2.1.1.182	malfunction	the enzyme knockout strain is sensitive to oxidative stress and has a lower survival rate in murine macrophage RAW264.7 cells than the parent strain	-, 733426	
2.1.1.182	malfunction	the inability to form dimethyladenine in the 16S rRNA-derived oligonucleotide is accompanied by muatation from kasugamycin sensitivity to resistance	705859	
2.1.1.182	malfunction	yeast enzyme Dim1 complements heterologously for ksgA- mutation in Escherichia coli, demonstrating functional equivalence of the two proteins	705108	
2.1.1.182	physiological function	knockout of KsgA attenuates the killing ability against silkworms. The KsgA knockout strain is sensitive to oxidative stress and has a lower survival rate in murine macrophages than the parent strain. The KsgA knockout strain exhibits decreased translational fidelity in oxidative stress conditions. Administration of N-acetyl-L-cysteine restores the killing ability of the knockout strain against silkworms	-, 756118	
2.1.1.182	physiological function	KsgA acts as a ribosome biogenesis factor. KsgA alters 16S rRNA processing and has a critical role is as a supervisor of biogenesis of 30S subunits in vivo	705774	
2.1.1.182	physiological function	KsgA confers kasugamycin sensitivity to Chlamydia trachomatis and impacts bacterial fitness	-, 702903	
2.1.1.182	physiological function	KsgA has a DNA glycosylase/AP lyase activity for C mispaired with oxidized T that prevents the formation of mutations, which is in addition to its rRNA adenine methyltransferase activity essential for ribosome biogenesis	705968	
2.1.1.182	physiological function	KsgA, in addition to its methyltransferase activity, has another unidentified function that plays a role in the suppression of the cold-sensitive phenotype of the Era(E200K) strain. The additional function may be involved in the acid shock response	704274	
2.1.1.182	physiological function	the enzyme contributes to maintain ribosome function under oxidative conditions and thus to Staphylococcus aureus virulence	-, 733426	
2.1.1.182	physiological function	the enzyme plays a role in intrinsic clarithromycin resistance and ribosome biogenesis in Mycobacterium tuberculosis	-, 733980	
2.1.1.182	physiological function	the KsgA methyltransferase is universally conserved and plays a key role in regulating ribosome biogenesis. KsgA has a complex reaction mechanism, transferring a total of four methyl groups onto two separate adenosine residues, A1518 and A1519, in the small subunit rRNA. This means that the active site pocket must accept both adenosine and N6-methyladenosine as substrates to catalyze formation of the final product N6,N6-dimethyladenosine	718932