1.14.99.38	malfunction	decitabine-induced hypomethylation of the cholesterol 25-hydroxylase gene is responsible for cell death of myelodysplasia/leukemia cells	746440	
1.14.99.38	malfunction	loss of function of Ch25h leads to susceptibility to viral infections in vitro	727611	
1.14.99.38	malfunction	mice with a disruption in the cholesterol 25-hydroxylase gene regulate cholesterol metabolism normally. Ch25h-/- mice have higher levels of mRNAs coding for immunoglobulin A and immunoglobulin J. Disruption in the Cyp7b1 gene leads to significantly increased levels of 25-hydroxycholesterol in the circulation, and to significantly lower levels of IgA compared to wild-type mice	727109	
1.14.99.38	malfunction	patients with hereditary spastic paresis, a rare inherited disease with mutations in the CYP7B1 gene, have highly elevated levels of 25- and 27-hydroxycholesterol but normal levels of cholesterol and bile acids	727109	
1.14.99.38	additional information	25-hydroxycholesterol does not stimulate the sPLA2-IIA promoter in the mouse Schwann cell line MSC80	727109	
1.14.99.38	additional information	Ch25h expression is controlled primarily by type I IFN signaling, type1 IFN-mediated Ch25h expression is IFNR-dependent, overview	-, 715833	
1.14.99.38	additional information	the high concentrations of vitamin D3 metabolites required for upregulation of the cholesterol 25-hydroxylase gene makes it unlikely that these metabolites are important regulators of cholesterol 25-hydroxylase in vivo	727109	
1.14.99.38	physiological function	adenovirus-mediated enzyme overexpression in mice improves glucose tolerance and insulin sensitivity and lowers the homeostatic model assessment for insulin resistance score	745450	
1.14.99.38	physiological function	cholesterol 25-hydroxylase acts as a host restriction factor on pseudorabies virus replication	745479	
1.14.99.38	physiological function	cholesterol 25-hydroxylase inhibits porcine reproductive and respiratory syndrome virus replication	745658	
1.14.99.38	physiological function	cholesterol 25-hydroxylase plays an essential role in cholesterol metabolism in the body by catalysing the formation of 25-hydroxycholesterol from cholesterol which acts to repress cholesterol biosynthesis	726545	
1.14.99.38	physiological function	cholesterol-25-hydroxylase acts as a broadly antiviral interferon-stimulated gene. CH25H converts cholesterol to a soluble antiviral factor, 25-hydroxycholesterol. 25-Hydroxycholesterol treatment in cultured cells broadly inhibits growth of enveloped viruses including VSV, HSV, HIV, and MHV68 and acutely pathogenic EBOV, RVFV, RSSEV, and Nipah viruses. The compound inhibits cell entry of viruses HIV and VSV, and inhibits viral protein-mediated cell-cell membrane fusion of virus NIV. 25-Hydroxycholesterol directly modifies membranes	727611	
1.14.99.38	physiological function	enzyme expression in human hepatoma cells restricts hepatitis C virus infection in a genotype-independent manner	745051	
1.14.99.38	physiological function	formation of 25-hydroxycholesterol in liver mitochondria is dependent on CYP27A1, and the 25-hydroxycholesterol formed is subsequently sulfated by the insulin-regulated cytosolic sulfotransferase 2B1b. 25-Hydroxycholesterol decreases the level of cytoplasmic IkBa and increases the nuclear NFkB level, its sulfated form has the opposite effects	727109	
1.14.99.38	physiological function	formation of 25-hydroxycholesterol in liver mitochondria is dependent on CYP27A1, and the 25-hydroxycholesterol formed is subsequently sulfated by the insulin-regulated cytosolic sulfotransferase 2B1b. 25-Hydroxycholesterol is, however, a weak activator of the liver X receptors. 25-Hydroxycholesterol is of great importance for maintaining cholesterol homeostasis. 25-Hydroxycholesterol induces the secretion of the proinflammatory cytokine IL-8	727109	
1.14.99.38	physiological function	important role for 25-hydroxycholesterol in the immune system. High concentrations of 25-hydroxycholesterol induce apoptosis in a mouse oligodendrocyte cell line 158N in an liver X receptor-independent manner. Treatment of these cells with 25-hydroxycholesterol results in enhanced secreted phospholipase A2 activity	727109	
1.14.99.38	physiological function	the enzyme is a host restriction factor and part of the primary innate immune responses against hepatitis C virus infection. The enzyme inhibits hepatitis C virus infection by suppressing the maturation of sterol regulatory element-binding proteins, critical transcription factors for host lipid biosynthesis	745657	
1.14.99.38	physiological function	the enzyme restricts hepatitis C virus replication not only through its enzyme activity to produce 25-hydroxycholesterol but also by targeting the NS5A protein leading to the selective inhibition of NS5A dimer formation	746435