2.3.1.74 down ability and effectiveness of four different viral suppressor proteins, i.e. the p38 protein of Turnip Crinkle Virus, the p25 protein of Potato Virus X, the 2b proteins of Cucumber Mosaic Virus and Tomato Aspermy Virus, that interfere with posttranscriptional gene silencing of the endogenous chalcone synthase gene in Arabidopsis thaliana when the silencing trigger and the viral suppressor protein are expressed from the same transgene locus. The silencing trigger consists of an inverted-repeat transgene construct that induces PTGS of the endogenous Arabidopsis thaliana CHS gene with high efficiency, overview 706186 2.3.1.74 down decrease at flowering 701213 2.3.1.74 down overexpression of the nuclear-localized protein MOS9 results in a reduction of enzyme transcript levels 757913 2.3.1.74 down the enzyme expression of isoform CHS06 is decreased in response to salicylic acid treatment 756987 2.3.1.74 down the mRNA expression of isoform CHS088 is decreased about 0.6fold by cold treatment (4°C for 3 h) or NaCl treatment (200 mM for 6 h). The mRNA expression of isoform CHS088 is decreased about 0.75fold by abscisic acid treatment (0.05 mM for 1 h) 755796 2.3.1.74 down the mRNA expression of isoform CHS444 is decreased about 0.3fold by cold treatment (4°C for 3 h) or 0.5fold by NaCl treatment (200 mM for 6 h). The mRNA expression of isoform CHS444 is decreased about 0.75fold by abscisic acid treatment (0.05 mM for 1 h) 755796 2.3.1.74 down the mRNA expression of isoform CHS768 is decreased about 0.6fold by cold treatment (4°C for 3 h) or NaCl treatment (200 mM for 6 h) 755796 2.3.1.74 down upon infection with hop stunt viroid, expression of chalcone synthase decreases up to 40fold with concomittant decrease in the expression of HlbHLH2 and an increase in the expression of HlMyb3 encoding transcription factors that form a ternary complex with WDR1 for CHS_H1 promoter activation 736691 2.3.1.74 additional information no change in the expression of CHS gene after leaf wounding treatment, overview 702519 2.3.1.74 additional information the mRNA expression of isoform CHS088 is not influenced by 20% (w/v) polyethylene glycol or 0.05 mM methyl jasmonate treatment 755796