3.4.21.97 755201 Dimerization-induced allosteric changes of the oxyanion-hole loop activate the pseudorabies virus assemblin pUL26N, a herpesvirus serine protease PLoS Pathog. 11 e1005045 2015 Suid alphaherpesvirus 1 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=26161660&form=6&db=m 3.4.21.97 755201 Dimerization-induced allosteric changes of the oxyanion-hole loop activate the pseudorabies virus assemblin pUL26N, a herpesvirus serine protease PLoS Pathog. 11 e1005045 2015 Suid alphaherpesvirus 1 Kaplan http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=26161660&form=6&db=m 3.4.21.97 95602 A new serine-protease fold revealed by the crystal strucutre of human cytomegalovirus protease Nature 383 272-275 1996 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8805706&form=6&db=m 3.4.21.97 649131 A rational approach towards successful crystallization and crystal treatment of human cytomegalovirus protease and its inhibitor complex Acta Crystallogr. Sect. D 56 175-180 2000 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=10666597&form=6&db=m 3.4.21.97 95605 Active human cytomegalovirus protease is a dimer J. Biol. Chem. 271 7445-7449 1996 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8631772&form=6&db=m 3.4.21.97 95599 Active site cavity of herpesvirus protease revealed by the crystal structure of Herpes simplex virus protease/inhibitor complex Biochemistry 36 14023-14029 1997 Herpes simplex virus http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9369473&form=6&db=m 3.4.21.97 95599 Active site cavity of herpesvirus protease revealed by the crystal structure of Herpes simplex virus protease/inhibitor complex Biochemistry 36 14023-14029 1997 Human alphaherpesvirus 1 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9369473&form=6&db=m 3.4.21.97 95592 Activity in monomers of human cytomegalovirus protease Biochem. Biophys. Res. Commun. 259 370-373 1999 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=10362516&form=6&db=m 3.4.21.97 95596 Assemblin, an essential herpesvirus proteinase Drug Des. Discov. 15 39-47 1997 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9332830&form=6&db=m 3.4.21.97 95593 beta-Lactam derivatives as inhibitors of human cytomegalovirus protease J. Med. Chem. 41 2882-2891 1998 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9667976&form=6&db=m 3.4.21.97 731318 Broad-spectrum allosteric inhibition of herpesvirus proteases Biochemistry 53 4648-4660 2014 Human gammaherpesvirus 4 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=24977643&form=6&db=m 3.4.21.97 731318 Broad-spectrum allosteric inhibition of herpesvirus proteases Biochemistry 53 4648-4660 2014 Human alphaherpesvirus 2 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=24977643&form=6&db=m 3.4.21.97 731318 Broad-spectrum allosteric inhibition of herpesvirus proteases Biochemistry 53 4648-4660 2014 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=24977643&form=6&db=m 3.4.21.97 731318 Broad-spectrum allosteric inhibition of herpesvirus proteases Biochemistry 53 4648-4660 2014 Human gammaherpesvirus 8 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=24977643&form=6&db=m 3.4.21.97 95604 Characterization of a soluble stable human cytomegalovirus protease and inhibition by M-site peptide mimics J. Virol. 70 4819-4824 1996 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8676515&form=6&db=m 3.4.21.97 95611 Characterization of the Epstein-Barr virus proteinase and comparison with the human cytomegalovirus proteinase J. Virol. 69 1265-1270 1995 Human gammaherpesvirus 4 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=7815503&form=6&db=m 3.4.21.97 95611 Characterization of the Epstein-Barr virus proteinase and comparison with the human cytomegalovirus proteinase J. Virol. 69 1265-1270 1995 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=7815503&form=6&db=m 3.4.21.97 650245 Characterization of the monomer-dimer equilibrium of human cytomegalovirus protease by kinetic methods Biochemistry 43 316-322 2004 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=14717585&form=6&db=m 3.4.21.97 95612 Characterization ot the proteinase specified by vircella-zoster virus gene 33 J. Gen. Virol. 78 2153-2157 1997 Human alphaherpesvirus 3 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9292001&form=6&db=m 3.4.21.97 665654 Chemical rescue of I-site cleavage in living cells and in vitro discriminates between the cytomegalovirus protease, assemblin, and its precursor, pUL80a J. Biol. Chem. 280 33206-33212 2005 simian cytomegalovirus http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=16036911&form=6&db=m 3.4.21.97 666188 Cleavage of human cytomegalovirus protease pUL80a at internal and cryptic sites is not essential but enhances infectivity J. Virol. 79 12961-12968 2005 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=16188998&form=6&db=m 3.4.21.97 95610 Cloning, expression, and characterization of the proteinase from human herpesvirus 6 J. Virol. 70 4136-4141 1996 Human betaherpesvirus 6 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8648756&form=6&db=m 3.4.21.97 95610 Cloning, expression, and characterization of the proteinase from human herpesvirus 6 J. Virol. 70 4136-4141 1996 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8648756&form=6&db=m 3.4.21.97 95586 Cytomegalovirus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 468-470 1998 Human betaherpesvirus 5 - 3.4.21.97 95586 Cytomegalovirus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 468-470 1998 simian cytomegalovirus - 3.4.21.97 653074 Cytomegalovirus assemblin (pUL80a): cleavage at internal site not essential for virus growth; proteinase absent from virions J. Virol. 76 8667-8674 2002 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=12163586&form=6&db=m 3.4.21.97 95607 Cytomegalovirus assemblin: the amino and carboxy domains of the proteinase form active enzyme when separately cloned and coexpressed in eukaryotic cells J. Virol. 70 5395-5404 1996 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8764050&form=6&db=m 3.4.21.97 718057 Cytomegalovirus capsid protease: Biological substrates are cleaved more efficiently by full-length enzyme (pUL80a) than by the catalytic domain (Assemblin) J. Virol. 85 3526-3534 2011 Cytomegalovirus http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=21270147&form=6&db=m 3.4.21.97 732540 Cytomegalovirus protease targeted prodrug development Mol. Pharm. 10 1417-1424 2013 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=23485093&form=6&db=m 3.4.21.97 652751 Design and synthesis of pyrrolidine-5,5'-trans-lactams (5-oxo-hexahydropyrrolo[3,2-b]pyrroles) as novel mechanism-based inhibitors of human cytomegalovirus protease. 4. Antiviral activity and plasma stability J. Med. Chem. 46 4428-4449 2003 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=14521407&form=6&db=m 3.4.21.97 652724 Design and synthesis of pyrrolidine-5,5-trans-lactams (5-oxo-hexahydro-pyrrolo[3,2-b]pyrroles) as novel mechanism-based inhibitors of human cytomegalovirus protease. 1. The alpha-methyl-trans-lactam template J. Med. Chem. 43 4452-4464 2000 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=11087570&form=6&db=m 3.4.21.97 652734 Design and synthesis of pyrrolidine-5,5-trans-lactams (5-oxohexahydropyrrolo[3,2-b]pyrroles) as novel mechanism-based inhibitors of human cytomegalovirus protease. 2. Potency and chirality J. Med. Chem. 45 1-18 2002 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=11754575&form=6&db=m 3.4.21.97 95600 Dimerization of the human cytomegaolvirus protease: kinetic and biochemical characterization of the catalytic homodimer Biochemistry 35 5300-5307 1996 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8611517&form=6&db=m 3.4.21.97 681686 Enzymatic activities of human cytomegalovirus maturational protease assemblin and its precursor (pPR, pUL80a) are comparable: [corrected] maximal activity of pPR requires self-interaction through its scaffolding domain J. Virol. 81 4091-4103 2007 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=17287260&form=6&db=m 3.4.21.97 95589 Epstein-Barr virus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 474-476 1998 Human gammaherpesvirus 4 - 3.4.21.97 95598 Evidence of a conformational change in the human cytomegalovirus protease upon binding of peptidyl-activated carbonyl inhibitors Biochemistry 36 12644-12652 1997 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9376371&form=6&db=m 3.4.21.97 95587 Herpesvirus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 470-472 1998 Bovine alphaherpesvirus 1 - 3.4.21.97 95587 Herpesvirus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 470-472 1998 Equid alphaherpesvirus 1 - 3.4.21.97 95587 Herpesvirus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 470-472 1998 equine herpesvirus type 2 - 3.4.21.97 95587 Herpesvirus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 470-472 1998 Herpes simplex virus - 3.4.21.97 95587 Herpesvirus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 470-472 1998 Human alphaherpesvirus 1 - 3.4.21.97 95587 Herpesvirus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 470-472 1998 Human alphaherpesvirus 2 - 3.4.21.97 95587 Herpesvirus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 470-472 1998 Saimiriine gammaherpesvirus 2 - 3.4.21.97 95587 Herpesvirus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 470-472 1998 Suid alphaherpesvirus 1 - 3.4.21.97 95608 Human cytomegalovirus maturational proteinase: expression in Escherichia coli, purification, and enzymatic characterization by using peptide substrate mimics of natural cleavage sites J. Virol. 68 2937-2946 1994 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8151764&form=6&db=m 3.4.21.97 681048 Human cytomegalovirus protease: Why is the dimer required for catalytic activity? J. Chem. Theory Comput. 3 278-288 2007 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=26627171&form=6&db=m 3.4.21.97 95588 Human herpesvirus type 6 assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 472-474 1998 Human betaherpesvirus 6 - 3.4.21.97 95595 Improved purification protocol of the HSV-1 protease catalytic domain, using immunoaffinity Biochem. Cell Biol. 75 795-801 1997 Human alphaherpesvirus 1 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9599670&form=6&db=m 3.4.21.97 95597 Independently cloned halves of cytomegalovirus assemblin An and Ac can restore proteolytic activity to assemblin mutants by intermolecular complementation J. Virol. 71 956-964 1997 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8995613&form=6&db=m 3.4.21.97 95603 Initial characterization of autoprocessing and active-center mutants of CMV proteinase J. Protein Chem. 15 763-774 1996 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9008301&form=6&db=m 3.4.21.97 650071 Investigating the role of histidine 157 in the catalytic activity of human cytomegalovirus protease Biochemistry 40 6344-6351 2001 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=11371196&form=6&db=m 3.4.21.97 653855 Investigation of the induced-fit mechanism and catalytic activity of the human cytomegalovirus protease homodimer via molecular dynamics simulations Proteins Struct. Funct. Genet. 52 483-491 2003 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=12910449&form=6&db=m 3.4.21.97 653320 Molecular mechanism for dimerization to regulate the catalytic activity of human cytomegalovirus protease Nat. Struct. Biol. 8 810-817 2001 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=11524687&form=6&db=m 3.4.21.97 677592 Novel yeast cell-based assay to screen for inhibitors of human cytomegalovirus protease in a high-throughput format Antimicrob. Agents Chemother. 50 565-571 2006 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=16436711&form=6&db=m 3.4.21.97 701149 Proteins related to green algal striated fiber assemblin are present in stramenopiles and alveolates Protoplasma 236 97-101 2009 Paramecium tetraurelia http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=19333716&form=6&db=m 3.4.21.97 701149 Proteins related to green algal striated fiber assemblin are present in stramenopiles and alveolates Protoplasma 236 97-101 2009 Phaeodactylum tricornutum http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=19333716&form=6&db=m 3.4.21.97 701149 Proteins related to green algal striated fiber assemblin are present in stramenopiles and alveolates Protoplasma 236 97-101 2009 Phytophthora infestans http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=19333716&form=6&db=m 3.4.21.97 701149 Proteins related to green algal striated fiber assemblin are present in stramenopiles and alveolates Protoplasma 236 97-101 2009 Phytophthora sojae http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=19333716&form=6&db=m 3.4.21.97 701149 Proteins related to green algal striated fiber assemblin are present in stramenopiles and alveolates Protoplasma 236 97-101 2009 Tetrahymena thermophila http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=19333716&form=6&db=m 3.4.21.97 701149 Proteins related to green algal striated fiber assemblin are present in stramenopiles and alveolates Protoplasma 236 97-101 2009 Thalassiosira pseudonana http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=19333716&form=6&db=m 3.4.21.97 701149 Proteins related to green algal striated fiber assemblin are present in stramenopiles and alveolates Protoplasma 236 97-101 2009 Phytophthora nicotianae http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=19333716&form=6&db=m 3.4.21.97 701149 Proteins related to green algal striated fiber assemblin are present in stramenopiles and alveolates Protoplasma 236 97-101 2009 Perkinsus marinus http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=19333716&form=6&db=m 3.4.21.97 701149 Proteins related to green algal striated fiber assemblin are present in stramenopiles and alveolates Protoplasma 236 97-101 2009 Phytophthora ramorum http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=19333716&form=6&db=m 3.4.21.97 95609 Purification and kinetic characterization of human cytomegalovirus assemblin Methods Enzymol. 244 417-423 1994 Human betaherpesvirus 5 - 3.4.21.97 95606 Purification of active Herpes simplex virus-1 protease expressed in Escherichia coli J. Biol. Chem. 269 18708-18711 1994 Human alphaherpesvirus 1 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8034621&form=6&db=m 3.4.21.97 95594 Recombinant human cytomegalovirus protease with a C-terminal (His)6 extension: purification, autocatalytic release of the mature enzyme, and biochemical characterization Protein Expr. Purif. 14 343-352 1996 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=9882568&form=6&db=m 3.4.21.97 732971 Release of the herpes simplex virus 1 protease by self cleavage is required for proper conformation of the portal vertex Virology 429 63-73 2012 Herpes simplex virus http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=22543049&form=6&db=m 3.4.21.97 650227 Structural and biochemical studies of inhibitor binding to human cytomegalovirus protease Biochemistry 42 885-891 2003 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=12549906&form=6&db=m 3.4.21.97 95591 Structure of the human cytomegalovirus protease catalytic domain reveals a novel serine protease fold and catalytic triad Cell 86 835-843 1996 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8797829&form=6&db=m 3.4.21.97 663617 Studies of substrate-induced conformational changes in human cytomegalovirus protease using optical biosensor technology Anal. Biochem. 332 203-214 2004 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=15325287&form=6&db=m 3.4.21.97 95601 Three-dimensional structure of human cytomegalovirus protease Nature 383 279-282 1996 Human betaherpesvirus 5 http://www.ncbi.nlm.nih.gov/htbin-post/Entrez/query?uid=8805708&form=6&db=m 3.4.21.97 95590 Varicella-zoster virus assemblin Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press - 476-478 1998 Human alphaherpesvirus 3 -