3.4.11.B8 The tetracosameric complex shows maximal activity against Ala-Ala-Ala and Ala-Ala-4-nitroanilide. Ala-Ala-Ala-4-nitroanilide is the best substrate for the dodecameric form of the enzyme. The enzyme is unable to cleave a peptidic bond when 4-nitroanilide or 7-amino-4-methylcoumarin is in the P1' position. - -