3.1.11.1 Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates mechanism 653308 3.1.11.1 Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates Preference for single-stranded DNA. The E. coli enzyme hydrolyses glucosylated DNA. Formerly EC 3.1.4.25. Similar enzymes: mammalian DNase III, exonuclease IV, T2- and T4-induced exodeoxyribonucleases - 3.1.11.1 Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates study of interaction between enzyme and mismatch repair proteins MLH1, MLH2 and MLH3. The enzyme binds strongly to MLH1 and forms an immunoprecipitable complex with MLH1/MLH2 in vivo 652161 3.1.11.1 Exonucleolytic cleavage in the 3'- to 5'- direction to yield nucleoside 5'-phosphates the enzyme is involved in mismatch repair and participates directly in somatic hypermutation and class-switch recombination 666421