2.7.1.232 ATP + 1,6-anhydro-beta-D-glucopyranose + H2O = ADP + 6-phospho-alpha-D-glucopyranose catalytic mechanism, molecular docking, dynamics simulation 738937 2.7.1.232 ATP + 1,6-anhydro-beta-D-glucopyranose + H2O = ADP + 6-phospho-alpha-D-glucopyranose mechanism of 1,6-anhydro bond cleavage, the enzyme binds levoglucosan in two distinct orientations, in addition to cleavage of the 1,6-anhydro ring, the pyranose ring of the levoglucosan is opened when the anomeric carbon of levoglucosan migrates from its position in a 1C4 to a 4C1 conformation, increased conformational strain during this reaction results in the linear form of G6P as the initial product, which then equilibrates to a mixture of both anomeric forms, structure-function analysis, detailed overview -, 738664 2.7.1.232 ATP + 1,6-anhydro-beta-D-glucopyranose + H2O = ADP + 6-phospho-alpha-D-glucopyranose the catalytic mechanism involves both cleavage of the 1,6-intramolecular linkage as well as phosphorylation 738435 2.7.1.232 ATP + levoglucosan + H2O = ADP + D-glucose 6-phosphate - -