1.1.1.363 D-glucose 6-phosphate + NAD(P)+ = 6-phospho-D-glucono-1,5-lactone + NAD(P)H + H+ - - 1.1.1.363 D-glucose 6-phosphate + NAD(P)+ = 6-phospho-D-glucono-1,5-lactone + NAD(P)H + H+ enzyme PputG6PDH-1 from Pseudomonas putida KT2440 catalyzes the oxidation of D-glucose 6-phosphate using a rapidequilibrium random mechanism -, 742533 1.1.1.363 D-glucose 6-phosphate + NAD(P)+ = 6-phospho-D-glucono-1,5-lactone + NAD(P)H + H+ mechanism in which His240 acts as the general base that abstracts the proton from the C1-hydroxyl group of glucose 6-phosphate, and the carboxylate group of D177 stabilizes the positive charge that forms on H240 in the transition state. The results also confirm the postulated role of His178 in binding the phosphate moiety of glucose 6-phosphate 721580