1.1.1.17 D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH + H+ - - 1.1.1.17 D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH + H+ the hydride transfer is strongly rate-determining for the overall enzymatic reaction 685964 1.1.1.17 D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH + H+ the phosphate moiety in Man-ol1P and Fru6P is essential for substrate recognition and/or catalysis by Aspergillus fumigatus M1PDH. Binding of D-mannitol 1-phosphate and NAD+ is random, whereas D-fructose 6-phosphate binds only after NADH has bound to the enzyme. Hydride transfer is rate-determining for D-mannitol 1-phosphate oxidation by AfM1PDH. The enzyme behaves kinetically as a fructose 6-phosphate reductase 722234