3.4.22.25 preferential cleavage: Gly-/-, in proteins and small molecule substrates - - 3.4.22.25 preferential cleavage: Gly-/-, in proteins and small molecule substrates specific for Gly at posotion P1 due to an exchange conserved residues Gly23 to Glu and Gly65 to Arg, 2 free thiol functions of cysteinyl residues are essential for the catalytic competence of the enzyme 652619