1.17.1.9 formate + NAD+ = CO2 + NADH + H+ hydride-transfer step is rate-limiting 669327 1.17.1.9 formate + NAD+ = CO2 + NADH + H+ ordered bi-bi mechanism, NAD+ is the first substrate and NADH is the last product 287875 1.17.1.9 formate + NAD+ = CO2 + NADH + H+ ordered BiBi mechanism with NAD+ and NADH being the first substrate and the second product, resp. 673381 1.17.1.9 formate + NAD+ = CO2 + NADH + H+ ordered kinetic mechanism with NAD+ adding before formate 288085 1.17.1.9 formate + NAD+ = CO2 + NADH + H+ ordered mechanism 288113 1.17.1.9 formate + NAD+ = CO2 + NADH + H+ reaction mechanism: step I, formate binds directly to Mo, displacing Se-Cys140. Step II, the alpha-proton from formate may be transferred to the nearby His141 that acts as general base. Alternatively, step II may involve a selenium-carboxylated intermediate. Step III, electrons from MoIV are transferred via the 4Fe-4S center to an external electron acceptor and the catalytic cycle is completed 674932 1.17.1.9 formate + NAD+ = CO2 + NADH + H+ sequential mechanism 288088 1.17.1.9 formate + NAD+ = CO2 + NADH + H+ sequential ordered bi-bi mechanism 288101