6.5.1.2	(deoxyribonucleotide)n-3'-hydroxyl + 5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP	(1c)	-	
6.5.1.2	ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond	1979, 649250, 649444, 650937, 650967, 651073, 651212, 651683, 652175, 652360, 652463, 652489, 652786, 653376, 653379, 653388, 653923	
6.5.1.2	ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	mechanism, the initial step is most likely a nucleophilic attack of the epsilon-amino group of a Lys on the adenylyl phosphorus of NAD+	1909, 1974	
6.5.1.2	ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	overall reaction	-	
6.5.1.2	ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide	the second step is the transfer of the AMP moiety from the enzyme to the 5'-phosphoryl of the DNA substrate, recreating a diphosphate linkage and thereby preserving the high-energy bond. The third step is the nucleophilic attack of the adjacent 3'-hydroxyl group to form a phosphodiester bond and eliminate AMP	1974	
6.5.1.2	NAD+ + [DNA ligase]-L-lysine = [DNA ligase]-N6-(5'-adenylyl)-L-lysine + beta-nicotinamide D-nucleotide	(1a)	-	
6.5.1.2	[DNA ligase]-N6-(5'-adenylyl)-L-lysine + 5'-phospho-(deoxyribonucleotide)m = 5'-(5'-diphosphoadenosine)-(deoxyribonucleotide)m + [DNA ligase]-L-lysine	(1b)	-