5.1.3.11 cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose a carbanion-type but no keto intermediate 2248 5.1.3.11 cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose direct reaction at carbon-2 of the reducing moiety of the disaccharide 2249 5.1.3.11 cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose mechanism of reversible epimerization involving three histidine residues, overview. His390 is closely related to ring opening/closure by transferring a proton between the O5 and O1 atoms of the ligand. His259 abstracts the H2 proton of the D-mannose residue at the reducing end, and consistently forms the cis-enediol intermediate by facilitated depolarization of the 2-OH group mediated by hydrogen bonding interaction with His200. His390 subsequently donates the proton to the C2 atom of the intermediate to form a D-glucose residue. The reverse reaction is mediated by the three histidines with the inverse roles of acid/base catalysts 727985 5.1.3.11 cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose reaction mechansim, overview -, 747318 5.1.3.11 cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose the enzyme's catalytic mechanism involvies three histidine residues, His374 and His243 act as a general acid/base catalyst, and His184 is essential for the enzyme activity, overview 727529