2.3.2.24	[(E3-independent) E2 ubiquitin-conjugating E2 enzyme]-S-monoubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	(1b)	-	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + [(E3-independent) ubiquitin-conjugating enzyme]-S-monoubiquitinyl-L-cysteine	(1a)	-	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues, the latter described for this enzyme. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11, as well as Lys48-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues, the latter described for this enzyme. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys48-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues, the latter described for this enzyme. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Polyubiquination occurs through all seven lysines of ubiquitin, with Lys11 as common as those of Lys48. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues, the latter described for this enzyme. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical polyubiquitin chains that are linked through Lys63	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues, the latter described for this enzyme. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. The UBE2V2/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys63. This type of poly-ubiquitination does not lead to protein degradation by the proteasome	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11-and Lys48-, as well as Lys63-linked polyubiquitination.	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes Lys11-linked polyubiquitination. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except Lys48-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer Lys11-, Lys27-, Lys48-, and Lys-63-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. In vitro catalyzes Lys11-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11- and Lys48-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11-, as well as Lys48- and Lys63-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys48-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes monoubiquitination and Lys48-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of Lys48-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Polyubiquination occurs through all seven lysines of ubiquitin, with Lys11 as common as those of Lys48	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Polyubiquination occurs through all seven lysines of ubiquitin, with Lys11 as common as those of Lys48. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Polyubiquination occurs through all seven lysines of ubiquitin. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys11- and Lys48-, as well as Lys63-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. Polyubiquination occurs through all seven lysines of ubiquitinAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys48-linked polyubiquitination	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	E2 enzymes autoubiquitinate both their active sites cysteine and lysine residues. E2 enzymes catalyze polyubiquitin formation with a specific lysine preference or a limited subset of lysine residues of ubiquitin. The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical Lys63-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome	725413	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	overall reaction	-	
2.3.2.24	[E1 ubiquitin-activating enzyme]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-N6-monoubiquitinyl-L-lysine	the enzyme forms a labile ubiquitin adduct in the presence of E1, ubiquitin, and MgATP and catalyzes the conjugation of ubiquitin to protein substrates, independent of E3	724254