6.3.3.2	-	
6.3.3.2	Ni-NTA affinity chromatography and gel filtration	
6.3.3.2	recombinant His-tagged enzyme 171fold to near homogeneity from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, the His-tag is removed by thrombin cleavage and dialysis	
6.3.3.2	recombinant His-tagged enzyme from Escherichia coli	
6.3.3.2	recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography	
6.3.3.2	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography to homogeneity	
6.3.3.2	recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography to over 95% homogeneity	
6.3.3.2	recombinant truncation mutant comprising residues 6-202 from Escherichia coli strain BL21(DE3) by affinity chromatography and substrate competition elution	
6.3.3.2	Talon metal affinity column chromatography	
6.3.3.2	Talon metal affinity resin chromatography and Sephadex G-25 gel filtration