6.3.2.8 - 6.3.2.8 method that includes chromatography using a Ni-nitrilotriacetic acid Superflow agarose-filled column and gel filtration 6.3.2.8 partial 6.3.2.8 purified with ProBond nickel-chelating resin 6.3.2.8 recombinant His-tagged enzyme BurB/CVs from Escherichia coli strain Rosetta (DE3) pLysS by nickel affinity chromatography, diaylsis, and ultrafiltration 6.3.2.8 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3), the His-tag is cleaved off by TEV protease 6.3.2.8 recombinant MurC from Escherichia coli strain JM83 by anion exchange chromatography 6.3.2.8 recombinant protein