6.3.1.13	2400fold, 2 peaks	
6.3.1.13	recombinant enzyme from mshC-deficient mutant strain I64 of Mycobacterium smegmatis by ammonium sulfate fractionation, anion exchange chromatography, hydroxyapatite chromatography, and gel filtration	
6.3.1.13	recombinant GST-tagged MshC and recombinant maltose binding protein-MshC by affinity chromatography on glutathione and amylose resins, respectively	
6.3.1.13	recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography and gel filtration to about 95% purity