6.2.1.2	-	
6.2.1.2	enzyme form HXM-A and HXM-B	
6.2.1.2	partial	
6.2.1.2	recombinant C-terminally His6-tagged maltose binding protein fusion from Escherichia coli by nickel affinity chromatography, the MBP is cleaved off by factor Xa, imidazole is eliminated by gel filtration, recombinant His-tagged mutants	
6.2.1.2	Sephadex G-25 gel filtration, DEAE-Sepharose column chromatography, and hydroxylapatite column chromatography