6.1.1.27	HisTrap column chromatography, HiTrap heparin column chromatography, and Superdex 200 gel filtration	
6.1.1.27	recombinant	
6.1.1.27	recombinant enzyme	
6.1.1.27	recombinant His-tagged enzyme from Escherichia coli by anion exchange chromatography, gel filtration, and nickel affinity chromatography	
6.1.1.27	recombinant His-tagged wild-type and mutant enzymes from Escherichia coli to homogeneity by nickel affinity chromatography, the His-tag is cleaved off	
6.1.1.27	TALON metal affinity resin column chromatography