5.4.4.2 - 492270, 492271, 492274, 492277 5.4.4.2 Co2+-ion affinity chromatography 690238 5.4.4.2 DEAE-Sepharose, Phenyl-Sepharose, Mono Q 652382 5.4.4.2 isolation of protoplasts 748946 5.4.4.2 Ni2+-charged HiTrap chelating agarose column chromatography and Superdex 75 gel filtration 705184 5.4.4.2 partial 492276 5.4.4.2 recombinant 660623 5.4.4.2 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography, gel filtration, and ultrafitration 726817 5.4.4.2 recombinant His6-tagged isozyme ICS1 from Escherichia coli strain Rosetta DE3 pLysS by nickel affinity chromatography and gel filtration 747030 5.4.4.2 recombinant His6-tagged isozyme ICS2 from Escherichia coli strain Rosetta DE3 pLysS by nickel affinity chromatography and gel filtration 747030