5.3.2.5 His-Bind resin column chromatography and Superose 6 gel filtration 714022 5.3.2.5 native enzyme by fractionation with help of protamine suzlfate, ammonium sulfate, and P-60, followed by anion exchange and hydrophobic interaction chromatography 669181 5.3.2.5 recombinant enzyme from Escherichia coli 701475 5.3.2.5 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 678268 5.3.2.5 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by two different steps of anion exchange chromatography and ultrafiltration 678268