5.3.1.24 - 5.3.1.24 165fold 5.3.1.24 21fold 5.3.1.24 a nickel Sepharose column, HisTrap FF, and a gel-filtration column, Sephacryl S200, are used 5.3.1.24 best isolated from mutant trp A2 5.3.1.24 component IIb of the anthranilate synthetase complex, purified from trp2 mutant 5.3.1.24 HisTrap FF nickel affinity column chromatography 5.3.1.24 indole-3-glycerol-phosphate synthetase/PRA isomerase complex 5.3.1.24 indole-3-glycerol-phosphate synthetase/PRA isomerase complex, 70fold 5.3.1.24 indole-3-glycerol-phosphate synthetase/PRA isomerase fragment of the multifunctional anthranilate synthetase complex beta subunit