5.1.3.7 - 5.1.3.7 by using nickel-nitrilotriacetic acid affinity chromatography and gel filtration 5.1.3.7 by using nickel-NTA affinity chromatography 5.1.3.7 MalE-Gne fusion protein 5.1.3.7 nickel-Sepharose column chromatography 5.1.3.7 recombinant enzyme 5.1.3.7 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity 5.1.3.7 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 5.1.3.7 recombinant N-termminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography