4.3.99.3 - 747084 4.3.99.3 a single step purification under anoxic conditions, using the His6 affinity tag step suffices to purify sufficient protein for biochemical, structural, and spectroscopic studies 748568 4.3.99.3 recombinant His-tagged QueE from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 717240 4.3.99.3 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 729229, 730439