4.2.2.19 purification based on affinity chromatography 4.2.2.19 recombinant enzyme comprising residues 25-506 overexpressed in Flavobacterium heparinum cells, to homogeneity 4.2.2.19 recombinant His-tagged enzyme from Escherichia coli, His-tag is removed, purified to homogeneity, 69fold 4.2.2.19 to homogeneity