4.1.2.20 - 4.1.2.20 ammonium sulfate precipitation, phenyl-Sepharose column chromatography, UnoQ5 column chromatography, and Superdex 200 gel filtration 4.1.2.20 DEAE-Sepharose column chromatography and Superdex 75 gel filtration 4.1.2.20 homogeneity 4.1.2.20 using metal affinity and size exclusion chromatography