3.8.1.2 - 648277, 648279, 648289, 648292, 648298, 648299, 648300, 648301, 648302, 648303, 648304, 648305, 648307, 648309, 723926, 756964, 757261 3.8.1.2 expression in Escherichia coli 667850 3.8.1.2 HiPrep 26/10 column chromatography, Super Q Toyopearl 650M column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration 695317 3.8.1.2 Ni-NTA column chromatography and gel filtration 697836 3.8.1.2 recombinant N-terminally His- and S-tagged enzyme from Escherichia coli strain BMRosetta by affinity purification followed by gel filtration, to homogeneity 735151 3.8.1.2 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)-RIPL by nickel affinity chromatography and gel filtration 733826 3.8.1.2 two-step method: heat treatment step that removes 90% of the impurities, followed by an acidification step that eliminates the remaining impurities. The easy purification procedure renders the production of this protein fast, cost effective, and suitable for large-scale production 723963