3.5.5.4 - 5824, 670571, 755848 3.5.5.4 ammonium sulfate precipitation and Ni-NTA column chromatography 689769 3.5.5.4 Ni-NTA agarose column chromatography and HiLoad 16/60 Superdex 200 gel filtration 688087 3.5.5.4 Ni-Sepharose Fast Flow column chromatography and Sephadex G-15 gel filtration 689084