3.4.22.52 - 3.4.22.52 affinity chromatography, calmodulin-like domain of the catalytic subunit expressed in E. coli 3.4.22.52 E-F hand structure domains 3.4.22.52 large scale 3.4.22.52 mu-calpain 3.4.22.52 Ni-NTA column chromatography 3.4.22.52 Ni2+-NTA-agarose column chromatography 3.4.22.52 partial 3.4.22.52 purification of a 21000 Da calpain small subunit fragment 3.4.22.52 single-step purification of calpain-1, calpain-2, and calpastatin using anion-exchange chromatography