3.4.21.B56 purification of the N-terminal fragment of PH1510 (1510-N, residues 16–236) expressed in Escherichia coli 722647 3.4.21.B56 recombinant His-tagged full-length enzyme from Escherichia coli strain BL21-CodonPlus by nickel affinity and anion exchange chromatography, followed by dialysis 731379