3.4.21.63 - 3.4.21.63 AP30 purified by cationic exchange resin followed by an affinity column, AP15 partially purified using an anion-exchange column 3.4.21.63 by ammonium sulfate precipitation, ion exchange chromatography and gel filtration 3.4.21.63 EI212 3.4.21.63 native enzyme 1.32fold by ammonium sulfate fractionation, and anion exchange chromatography 3.4.21.63 native enzyme 28.6fold by acetone precipitation, freezing of the precipitate for 3 h at -20°C, dissolvation, and lyophilization, followed by concanavalin A lectin affinity chromatography 3.4.21.63 native enzyme by anion exchange chromatography 3.4.21.63 native enzyme partially by acetone precipitation 3.4.21.63 native extracellular enzyme 1.38fold from strain A10 by ammonium sulfate fractionation, dialysis, and anion exchange chromatography 3.4.21.63 native extracellular enzyme 7fold from strain M30 by ammonium sulfate fractionation, hydrophobic interaction chromatography, and anion exchange chromatography