3.4.19.13 - 754222, 755326 3.4.19.13 for crystallization studies, the natural variant V272A of the human enzyme is expressed in Pichia pastoris strain X-33, purified, and deglycosylated 754112 3.4.19.13 native enzyme 40.7fold from leaves by ammonium sulfate fractionation, dialysis, and gel filtration 757351 3.4.19.13 recombinant extracellular C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography 757413 3.4.19.13 recombinant protein 723208