3.2.1.68	-	
3.2.1.68	ammonium sulfate precipitation, DEAE cellulose column chromatography, CM-cellulose column chromatography, and DEAE-Sephadex gel filtration	
3.2.1.68	ammonium sulfate precipitation, Ni-NTA column chromatography, and Superdex 200 gel filtration	
3.2.1.68	copurification of native isozymes ISA1 and ISA2 partially from leaves by gel filtration, copurification of TAP-tagged ISA1 and ISA2 from Escherichia coli strain BL21(DE3) by tandem-affinity chromatography on TEV and calmodulin, followed by gel filtration	
3.2.1.68	HiTrap chelating column chromatography	
3.2.1.68	HiTrapQ column chromatography and Superdex 200 gel filtration	
3.2.1.68	HitrapQ column chromatography and TSKgel DEAE-5PW column chromatography	
3.2.1.68	HitrapQ column chromatography and TSKgel DEAE-5PW gel filtration	
3.2.1.68	native extracellular enzyme 369fold from culture supernatant by ultrafiltration, ammonium sulfate fractionation, gel filtration, two different steps of anion exchange chromatography, and dialysis, to homogeneity	
3.2.1.68	native subunit ISA2 from leaves by anion exchange chromatography, ultrafiltration, and gel filtration, separation from subunit ISA1