3.2.1.135	-	
3.2.1.135	ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Sephadex G-100 gel filtration	
3.2.1.135	BD TALON spin column for 6x His-tag protein affinity purification, simultaneous SDS-PAGE zymogram with 1% soluble starch	
3.2.1.135	gene purification by agarose gel electrophoresis, enzyme purification by size exclusion chromatography, elution via Superdex 200 10/300 GL column with 50 mM MOPS, pH7, 50 mM NaCl	
3.2.1.135	Lactococcus lactis cytoplasmic fraction containing the alpha-amylase and pullulanase domain proteins, respectively, is purified by using Ni-nitrilotriacetic acid matrices, elution fractions analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis	
3.2.1.135	recombinant enzyme	
3.2.1.135	supernatant ultrafiltrated, DEAE Sepharose CLl-4B, Superose 6