3.10.1.1 - 3.10.1.1 apparent homogeneity 3.10.1.1 partial purification 3.10.1.1 purification of recombinant enzyme 3.10.1.1 recombinant 3.10.1.1 recombinant C-terminally His6-tagged full-length enzyme from HEK-293 cells by nickel affinity croomatography 3.10.1.1 SP-Sepharose column chromatography and Sephacryl S-300 gel filtration