3.1.21.3	-	
3.1.21.3	C.Esp1396I-6His and C.Esp1396I without hexahistidine tag	
3.1.21.3	EcoR124I methylase is purified from Escherichia coli strain JM109(DE3) harboring plasmid pJS4M	
3.1.21.3	enzyme EcoB	
3.1.21.3	enzyme EcoK	
3.1.21.3	expression in Escherichia coli	
3.1.21.3	HisTrap column chromatography, DEAE column chromatography, and heparin column chromatography	
3.1.21.3	HsdM subunit, on anion-exchange column, more than 95% pure	
3.1.21.3	HsdR fusion protein purified by gel filtration. HsdR with five additional amino acids (YFQGA) at the N-terminus purified on anion-exchange column and by gel filtration, purified protein is more than 95% pure	
3.1.21.3	mixture of two enzyme species, the larger species has the stoichiometry R2M2S1, the smaller species has the stoichiometry of R1M2S1.only the R2M2S1 complex is capable of DNA cleavage, the R1M2S1 complex retains ATPase activity