2.7.6.5 - 2.7.6.5 affinity-chromatography and precipitation 2.7.6.5 HiTrap column chromatography 2.7.6.5 Ni-NTA column chromatography, and gel filtration 2.7.6.5 recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography and gel filtration 2.7.6.5 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration 2.7.6.5 the catalytic fragment RelSeq 1-385